Identification of P2RY8-CRLF2 in childhood BCP-ALL cases. (A) PCR products of the genomic P2RY8-CRLF2 fusion of representative cases. Patient identification is indicated at the top of the gel: ad, nontemplate control; pB, peripheral blood MNCs; and SM, size marker. Left part of the gel (samples 360-903) shows PCR products from cases that were later defined as harboring the P2RY8-CRLF2 fusion in a major clone. Right part of the gel (samples 460-906) shows PCR products from ALL cases with P2RY8-CRLF2 in a minor subclone. (B) PCR for the fusion transcripts of corresponding cases. Note that sample 365 had no genomic PCR product but a distinct band for the transcript. (C) PCR products of the 6 cases with the newly discovered breakpoints upstream of CRLF2. A vertical line was inserted to indicate that different gels, run in parallel, from identical experiments were pasted together.