SINEs antagonize microenvironment stimuli. CD19⁺ cells from CLL patients (N = 10) were incubated with or without 1μM KPT-185 or KPT-251 for 72 hours in presence or absence of (A) 20 ng/mL TNF, (B) 40 ng/mL IL-6, (C) 800 U/mL IL-4, (D) 3μM of CPG, (E) 1 μg/mL CD40L, and (F) 50 ng/mL BAFF. (G) CD19⁺ cells from CLL patients were isolated from peripheral blood and incubated with or without KPT-185 or KPT-251 (1 and 2.5μM) in suspension or on an HS5 cell layer for 48 hours. Viability was determined by annexin-V/PI flow cytometry, and is shown relative to time-matched DMSO controls for each group. Red circles represent averages. (H) CD19⁺ cells from CLL patients were isolated from peripheral blood and incubated with or without KPT-185 or KPT-251 (1 and 2.5μM) for 12 hours. Drug was then washed out and cells were incubated in suspension or on an HS5 cell layer for additional 48 hours. Viability was determined by annexin-V/PI flow cytometry, and is shown relative to time-matched DMSO controls for each group. Horizontal bars represent averages.