Induction of CCR8 expression by cutaneous DCs. Purified allogeneic naive T cells were cocultured for 9 days with MACS-enriched epidermal LCs, dermal CD1a⁺ DCs, dermal CD14⁺ DCs, or mature MDDCs at a ratio of 10:1. (A) Representative dot plots for CCR8 expression on gated CD3⁺ T cells. (B-C) Combined expression data for CCR8 and CXCR3 from 2-6 independent experiments. Horizontal lines indicate the median; and +, the mean. **P < .01, comparing T cells stimulated with LCs versus MDDCs. (D) The percentage of CD3⁺ T cells expressing CCR8 after coculture for 9 days with epidermal LCs from each donor (n = 12) is plotted against LC purity as defined by the percentage of epidermal cells expressing CD207 (Langerin) after MACS enrichment.