Functional avidity of transduced T cells expressing γ9δ2TCR G115 with γ9-CDR3 length mutations. (A) Peripheral blood T cells were virally transduced with indicated γ9 and δ2 TCR chains. Lytic activity of transductants was compared side-by-side with T cells expressing individual γ9δ2TCRs of the same γ9CDR3 length. Specific lysis is indicated as the fold change ⁵¹Cr-release measured in the supernatant after 5 hours. Data represent the means ± SD. **P < .01 by 1-way ANOVA. (B) Crystal structure of γ9δ2TCR G115 indicating γ9CDR3 in gray including amino acids γ9-G115_A109, γ9-G115_Q110, andγ9-G115_Q111 (red arrows). δCDR3 is shown in green; the δ chain in blue; and the γ chain in brown.