BAF53a deletion results in depletion of the HSC and progenitor pools. (A) Representative FACS profiles of BM HSC, CMP, GMP, and MEP populations in BAF53a^fl/flCre⁺ mice on day 14 after pIpC-induced deletion. (B) Absolute number of BM CMPs, MEPs, and GMPs per femur in animals treated as in panel A. Data are mean ± SD; n > 5 per genotype. (C) Absolute number of BM myeloid CFCs in pIpC-treated BAF53a^fl/flCre⁺ mice (day 14). n = 4 per genotype. CFU-GEMM indicates colony-forming unit-granulocyte, erythrocyte, monocyte and megakaryocyte; CFU-GM, colony-forming unit-granulocyte monocyte; CFU-G, colony-forming unit-granulocyte; and CFU-M, colony-forming unit-macrophage. (D) Representative FACS profiles of LT-HSCs isolated from the BM of pIpC-treated BAF53a^fl/flCre⁺ mice (day 14). Lineage, Sca1, CD150, and CD48 antibodies were used. The use of c-kit antibodies would erroneously demonstrate a total loss of LT-HSCs. Therefore, c-kit labeling was deliberately excluded from the analysis. (E) Absolute number of LT-HSCs per femur in pIpC-treated BAF53a^fl/flCre⁺ animals (day 14). n = 5 per genotype. (F) Absolute number of Sca1⁺Lin⁻c-kit⁺ (KLS) cells per spleen in pIpC-treated BAF53a^fl/flCre⁺ animals (day 4). n = 2 per genotype. **P ≤ .01 (Student t test).