JAG2 induces MMP expression in MoDCs. (A) GM-CSF– and IL-4–generated MoDCs were cocultured with JAG2 or control transfected MS5 feeder cells for 15 hours. As a benchmark for protease induction, TNFα was added instead of JAG2 or control feeder cells. Cells were harvested and analyzed for induction of MMP1, MMP12, and ADAMDEC1. Transcripts were normalized to β₂-microglobulin and induction over control transfected feeder cells is shown. Feeder cells alone did not show significant induction of transcripts. Error bars show the SD. The results of 1 of 6 independent experiments with 3 different donors are shown. (B) Induction of MMP1 expression in monocytes and MoDCs. Cells were plated onto feeder cells expressing JAG2 or control cells and harvested after 14 hours. MMP1 RNA induction was normalized to β₂-microglobulin and relative expression values are shown. The results of 1 of 3 independent experiments with 5 different donors are shown.