Figure 5
![Figure 5. Exogenous PIP2 promotes ectopic angiogenesis in wild-type zebrafish in vivo. (A-C) Confocal images of subintestinal vessels (SIVs) extending over the yolk cell of 3 days postfertilization (dpf) Tg(fli-EGFP)y1 zebrafish that were untreated (A), received a single intravascular injection of carrier-loaded phosphatidylinositol-4,5-bisphosphate [(PI(4,5)P2] at 2 dpf (B), or received an injection of CMV:vegfaa at the 1-cell stage (C). Lateral views, anterior to the left. (D-F) Quantitation of the average number of ectopic SIV ventral sprouts (D), average total length of ectopic SIV ventral sprouts (E), and average width of the normal SIV plexus (F) in 3-dpf embryos. Scale bars = 200 μm (A-C). EGFP indicates enhanced green fluorescent protein.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/120/2/10.1182_blood-2012-02-408328/4/zh89991293850005.jpeg?Expires=1724148037&Signature=5P1MBXX9OpqLOniYdmtBjWWaQDfJnumGnmEHEG5fvjnoP-jQlPknFBQ5a2RhwSZKn0IJhE6F~AeAJ2NKKLBiuJBBKHS~lXSUSIhzg1JKnaflbUpFz2kgo-I-hKaa5YKFBjDcZh55ywQhUZBtgeRD~foGRYVRnfl81U-6RY5ju7dDFYZZHtkKD7mZf62a2gi2N~HAg5TVvJ9-QEqSd5ZtNF~G3XIhg1zD7vYdxEh6UJPj2k9FwTo66OfFW3zZvfIcnarIXSEM~cob8W~U8Tmu2u2dvfRK2E7p2~Q2Ra8hIPKSTnS7SNOxQ5xwa8aMI85wrTRchziSaQdtVtCXC7y6~A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Exogenous PIP2 promotes ectopic angiogenesis in wild-type zebrafish in vivo. (A-C) Confocal images of subintestinal vessels (SIVs) extending over the yolk cell of 3 days postfertilization (dpf) Tg(fli-EGFP)y1 zebrafish that were untreated (A), received a single intravascular injection of carrier-loaded phosphatidylinositol-4,5-bisphosphate [(PI(4,5)P2] at 2 dpf (B), or received an injection of CMV:vegfaa at the 1-cell stage (C). Lateral views, anterior to the left. (D-F) Quantitation of the average number of ectopic SIV ventral sprouts (D), average total length of ectopic SIV ventral sprouts (E), and average width of the normal SIV plexus (F) in 3-dpf embryos. Scale bars = 200 μm (A-C). EGFP indicates enhanced green fluorescent protein.
