Identification of Meis1 and Meis2 target genes. (A) A2lox.Meis1 or A2lox.Meis2 ES cells were differentiated as in Figure 3A with or without treatment with doxycycline and were purified by cell sorting on day 7 or day 8 into the indicated populations based on levels of CD41 expression: CD41⁻, CD41⁺ (day 7) and CD41⁻, CD41^int, and CD41^hi (day 8). (B-C) Gene expression of A2lox.Meis2-derived populations with (+) or without (−) treatment by doxycyline (dox) was determined by microarray analysis. (B) Hemoglobin α (Hba-a1/2) and glycophorin A (Gypa) expression is shown for the indicated populations treated with (+) or without (−) dox. (C) Expression of placenta-specific 8 (Plac8), serine peptidase inhibitor, clade B, member 2 (Serpinb2), prostaglandin E receptor 3 (Ptger3), and platelet factor 4 (Pf4) is shown for the indicated populations and conditions. (D) Expression of Plac8 and Ptger3 by CD41^hi day 8 cells treated with (+) or without (−) dox as described in panel A was determined by quantitative RT-PCR.