Adaptation and survival of RBCs with reduced surface area and S/V ratio during the isolated-human spleen perfusion. Dimension (A-C) and morphology (D-E) analysis of LPC-treated RBCs before (T0) and 40 minutes (T40) after the start of the perfusion. Projected surface area (A), diameters (B), and perimeters (C) of LPC-treated RBCs were similar between T0 and T40, and all were significantly lower than that of untreated RBCs. Shifts toward normal values of the circularity (D) and the aspect ratio or sphericity (E) of LPC-treated RBCs at T40. See also supplemental Figure 3 for the deformability profile of LPC-treated RBC populations before and the kinetics of their clearance during 60 minutes of spleen perfusion. Projected surface area is in square micrometers; diameter and perimeter are in micrometers. One of 3 representative experiments is shown.