Monitoring of LPC-treated RBC features. (A) Osmotic fragility test of LPC-treated RBCs (1 of 3 representative experiments). (B) Linear regression fit of the correlation between NaCl concentration inducing 50% of hemolysis and the extent of surface area loss (a representative experiment). There was a good correlation (r = 0.998; P < .0001) between the extent of surface area loss and the osmolarity value at which 50% of RBCs hemolyze. (C) Deformability profiles of LPC-treated RBCs used for splenic perfusion studies (a representative experiment). The RBC deformability changes that occurred after LPC treatment were examined with the laser-assisted optical rotational cell analyzer, a system that measures the extent of cell deformation as a function of applied shear stress. LPC-treated RBCs were less deformable than control/untreated RBCs, with a dose dependent reduction in EI_max, a deformability parameter. (D) Linear regression fit of the correlation between EI_max and the extent of surface area loss.