Effects of LPC treatment on RBC membrane surface area and cell morphology. Analysis of RBC dimension, morphology, and morphologic homogeneity using ImageStream technology. There is a dose-dependent shift toward lower values of the distribution of RBC membrane surface area (A), whereas the aspect ratio (sphericity; B) increased with increasing concentrations of LPC. (C) The RBC populations are morphologically homogenous even at low LPC concentrations, as characterized by the RBC distribution shifting as a whole. RBCs with a circular shape in the control group did not correspond to spherical cells, but were instead discocytes, because the images were captured face on.