Figure 4
NK cell number is rescued in chimeric mice. (A) Diagram of chimera generation. SHIP+/− or SHIP−/− (CD45.1+) bone marrow cells were transplanted into lethally irradiated WT (CD45.2+) recipients and analyzed 4 to 9 weeks after reconstitution. (B) Percentage and total NK cells (NK1.1+CD3− in the lymphocyte gate) present in the indicated organs from SHIP+/− chimera (gray circles) and SHIP−/− chimera (black circles) mice. Data are pooled from at least 5 independent experiments, and each symbol indicates an individual mouse; horizontal lines indicate the mean. Data are pooled from at least 5 independent experiments, and each symbol indicates an individual mouse; horizontal lines indicate the mean. (C) Percentage of donor contributions of NKPs (CD122+NK1.1−CD3−CD11b−), iNK cells (CD122+NK1.1+CD3−CD11b−), and mNK cells (CD122+NK1.1+CD3−CD11b+) among bone marrow lymphocytes derived from SHIP+/− chimeras (gray circles) and SHIP−/− chimeras (black circles) mice. Data are pooled from at least 5 independent experiments, and each symbol indicates an individual mouse; horizontal lines indicate the mean. (D) Percentages in NK cells (NK1.1+CD3− in the lymphocyte gate) expressing DN (CD27−CD11b−), CD27 SP (CD11b−CD27+), DP (CD27+CD11b+), and CD11b SP (CD27−CD11b+) populations in the indicated organs derived from SHIP+/− chimera (gray bars) and SHIP−/− chimera (black bars) mice. Data are pooled from at least 5 independent experiments. Error bars indicate SEM.

NK cell number is rescued in chimeric mice. (A) Diagram of chimera generation. SHIP+/− or SHIP−/− (CD45.1+) bone marrow cells were transplanted into lethally irradiated WT (CD45.2+) recipients and analyzed 4 to 9 weeks after reconstitution. (B) Percentage and total NK cells (NK1.1+CD3− in the lymphocyte gate) present in the indicated organs from SHIP+/− chimera (gray circles) and SHIP−/− chimera (black circles) mice. Data are pooled from at least 5 independent experiments, and each symbol indicates an individual mouse; horizontal lines indicate the mean. Data are pooled from at least 5 independent experiments, and each symbol indicates an individual mouse; horizontal lines indicate the mean. (C) Percentage of donor contributions of NKPs (CD122+NK1.1−CD3−CD11b−), iNK cells (CD122+NK1.1+CD3−CD11b−), and mNK cells (CD122+NK1.1+CD3−CD11b+) among bone marrow lymphocytes derived from SHIP+/− chimeras (gray circles) and SHIP−/− chimeras (black circles) mice. Data are pooled from at least 5 independent experiments, and each symbol indicates an individual mouse; horizontal lines indicate the mean. (D) Percentages in NK cells (NK1.1+CD3− in the lymphocyte gate) expressing DN (CD27−CD11b−), CD27 SP (CD11b−CD27+), DP (CD27+CD11b+), and CD11b SP (CD27−CD11b+) populations in the indicated organs derived from SHIP+/− chimera (gray bars) and SHIP−/− chimera (black bars) mice. Data are pooled from at least 5 independent experiments. Error bars indicate SEM.

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