Increase of IFN-γ can induce CXCR3 expression on AFCs of all Ig isotypes. (A) Chimeras constructed by transfer of OTII cells or both OTI and OTII cells were immunized with alumOVA in both footpads. Seven days after immunization, suspensions of cells from the 2 popliteal LN of each mouse were assessed by flow cytometry to determine the proportions of CD138⁺B220^int AFCs of each Ig isotype that expressed CXCR3. The data are derived from 2-5 independent experiments with a total of 8-12 mice in each group. (B) Chimeras were constructed with OTI and OTII cells and immunized in the presence of neutralizing anti–IFN-γ antibody or isotype control antibody, as explained in Figure 2. Seven days after immunization, the proportions of CD138⁺B220^int AFCs of each Ig isotype that expressed CXCR3 were compared by flow cytometry in the draining LNs of the chimeras treated with anti–IFN-γ or with isotype control antibody. Data are derived from 2 independent experiments with a total of 8 mice per group. The significance of differences between groups is assessed by 2-tailed Mann-Whitney nonparametric statistics.