Reduced total but normal antigen-specific antibodies and IgG memory B-cell responses in CGD patients. PBMCs or CD27-fractionated B cells were cultured for 4 days with SAC and CpG and evaluated for IgG ASC frequencies by Elispot using various antigens to capture ASCs and detection with antihuman IgG. (A) Total IgG ASC frequencies were measured by coating wells with antihuman λ and κ light chain antibodies. (B) IgG ASC frequencies specific for seasonal (left panel) or H1N1 (right panel) were measured by coating wells with respective influenza vaccine preparations. (C) H1N1 influenza-specific serum antibody titers were measured by microneutralization assay. (D) Influenza-specific ASCs were calculated and reported as a fraction of the total ASC response shown in panel A. Horizontal bars represent medians.