Distinct SHM profiles for memory CD27⁻IgG⁺ and CD27⁺IgG⁺ B cells. The CD19⁺/CD3⁻ cells of PBMCs from one HD and 3 CGD patients were sorted into CD27⁻IgG⁺ and CD27⁺IgG⁺ fractions and mutation frequencies were calculated for (A) IGHV, (B) IGKV, and (C) IGLV genes of each fraction. The number of clones sequenced from each subset/donor is shown in parentheses below the graphs. Numbers in the CGD identifiers below the graphs correspond to the patients studied (Table 1). Horizontal bars represent medians.