Reduced levels of Th-POK reporter gene expression in NKT17 cells from WT mice. (A,C) Histogram overlays depicting GFP expression in the indicated subpopulations from the (A) peripheral LN or (C) thymus of a Th-POK^GFP/+ mouse. (B,D) Bar graphs of the arithmetic mean of the relative GFP MFI of the indicated populations from the (B) peripheral LN or (D) thymus of 2 Th-POK^GFP/+ mice. MFI were normalized to the levels in (B) peripheral LN CD4 SP or (D) mature CD4 SP thymocytes. Data are representative of 4 mice analyzed in 2 separate experiments. P values are shown for comparisons of selected populations in all of the analyzed samples, calculated using a paired Student t test of the GFP MFI after log transformation.