Figure 4
Effect of calpain activation on ILK. (A) Effect of Ca2+ with or without calpeptin (Cpt, 10μM) on the association of PINCH and α-Parvin with ILK. (B) Effect of purified μ- and m-calpain on the ability of PINCH-ILK complexes to phosphorylate Akt (on Ser-473) in washed human platelets. (C) Consequences of calpain activation by ionomycin (Iono, 1μM) on the levels of ILK, α-Parvin, and PINCH in washed human platelets. Identical results were obtained in 4 additional experiments. The graphs summarize data obtained with 5-6 subjects per group. *P < .05 versus CTL/Sol.
