CD4⁺ and CXCR3⁺ T cells are elevated in the skin of SCT patients with cGVHD. Shown are the results of immunohistochemical analysis of CD4 and CXCR3 tissue distribution in control healthy skin and in SCT patients with cGVHD. Paraffin-embedded, antigen-retrieved tissue sections were incubated with CD4 (A) and CXCR3 (B) mAbs plus secondary anti–mouse/rabbit HRP. Staining was developed with DAB chromogen and sections counterstained with hematoxylin. Images were taken at room temperature on a Zeiss Axioscop 40 microscope with a Zeiss AxioCam MRc5 camera, magnification 40×/0.65 air. Images were acquired with AxioCam Version 4.4 software, and analyzed using ImageJ Version 1.46 software.