Subcapsular macrophages are required for NK cell recruitment in the SCS area and deceleration. NCR1^GFP/+ mice were treated with clodronate (CL-Lipo) or PBS and injected with MVA in the footpad. After 24 hours, intact popliteal lymph nodes were subjected to two-photon imaging. (A-B) Representative images showing that NK cell accumulation in the SCS area of the lymph node in response to MVA is lost in CL-Lipo treated mice. (C-D) DISC analyses showing the location and speed of NK cells in control and Lipo-CL treated mice injected with MVA.