MVA induces NK cell accumulation in the subcapsular area of the lymph node. NCR1^GFP/+ mice were injected in the footpad with 5 × 10⁶ pfu of MVA or with PBS. After 16 hours, intact popliteal lymph nodes were subjected to two-photon imaging. (A-B) Representative images from PBS (A) or MVA (B) treated mice. NK cells are shown in green and collagen (2nd harmonic generation) in red. The outline of the lymph node is shown by a dashed line. (C-D) DISC profiles showing the localization of NK cells in PBS (C) or MVA-treated (D) mice and their speed after gating on the indicated area. (E) NCR1^GFP/+ mice were injected in the footpad with 5 × 10⁶ pfu of MVA or with PBS. At 16 hours, FITC-labeled Fab fragments from an anti-CD169 mAb were injected in the footpad for in vivo labeling of CD169⁺ SCS macrophages and 1 hour later popliteal lymph nodes were subjected to two-photon imaging. Representative images from PBS and MVA-treated mice (left and middle). NK cells are shown in red, CD169⁺ cells in green and collagen in white. Tracks of NK cells in MVA treated mice are shown in red (right).