Local administration of MVA induces NK cell recruitment in the draining lymph node. WT (C57BL/6), CXCR3^−/− or CCR5^−/− mice were injected in the footpad with 5 × 10⁶ pfu of MVA or with PBS. After 16 hours, cells from the draining popliteal lymph node were analyzed by flow cytometry. (A) NK cell accumulation after MVA injection. Profiles are gated on CD3⁻ cells. Numbers correspond to percentage of cells falling in the indicated gate. (B) The absolute numbers of NK cells in the draining lymph node is graphed for WT, CXCR3^−/−, or CCR5^−/− mice. (C) The percentage of NK cells (NK1.1⁺CD3⁻) is graphed for WT, CXCR3^−/−, or CCR5^−/− mice. Data are representative of 3 independent experiments (**P < .01).