Slc35d3 expression is up-regulated during megakaryocytoid differentiation. (A) Quantitative real-time RT-PCR analysis of mRNA from undifferentiated FLCs (day 0) and FLC-derived MKs (FLC-MK) isolated 8 days after stimulation in culture with Tpo. mRNA levels for Slc35d3 and Mrp4 are normalized to 1 for FLC and internally controlled by Gapdh levels. (B) Immunoblot analysis of indicated cells or cell lines with affinity-purified anti-SLC35D3 Ab (top) or anti-actin Ab (bottom) as a control. Mel and Mel-SLC35 represent melan-Ink4a melanocytic cells that were untreated or transduced with retrovirus to express mouse SLC35D3 as negative and positive controls, respectively. Plt indicates platelets isolated from C57BL/6J mice. Only the relevant region of each gel is shown. Positions of molecular weight markers are shown on the left, and the specific SLC35D3 and actin bands are indicated by lines on the right.