KNG deficiency reduces inflammation after stroke. (A) Relative gene expression of Il-1β, Tnf-α, and Tgf-β1 in the cortices and basal ganglia of wild-type (WT) mice and Kng^−/− mice 24 hours after tMCAO or sham operation (N = 4 per group). (B) Cellular inflammatory response in the ischemic hemispheres of WT mice or Kng^−/− mice on day 1 and day 3 after tMCAO. (Top) Representative immunohistochemical staining against CD11b⁺ macrophages/microglia cells (arrows) on day 1. Scale bar represents 100 μm. (Bottom) Quantification of immune cell infiltration (CD11b⁺ macrophages/microglia, Ly-6B²⁺ neutrophils) on day 1 and day 3 after stroke (N = 5 per group and time point). ***P < .001, **P < .01, ^###P < .001, ^#P < .05, 2-way ANOVA, followed by Bonferroni multiple comparison test compared with WT mice on day 1 (* symbol) or WT mice on day 3 (^# symbol).