KNG deficiency provides sustained protection from ischemic stroke and does not increase the risk of intracranial hemorrhage. (Top) Serial coronal T2-weighted gradient echo MR images show extensive hyperintense (bright) ischemic lesions (white arrows) on day 1 and day 7 after tMCAO in wild-type (WT) mice, whereas the infarctions are mainly restricted to the basal ganglia in Kng^−/− mice. Hypointense (dark) areas indicative of intracerebral hemorrhage were always absent in both groups. One representative panel per group is depicted. (Bottom) MRI-based infarct volumetry confirms significantly smaller infarct volumes and excludes delayed infarct growth in Kng^−/− mice after tMCAO (N = 3-8 per group and time point). Alleged shrinkage of strokes between day 1 and day 7 is because of fogging effects during infarct maturation. Note that 5 of 8 WT mice died between day 1 and day 7 after tMCAO and were no longer available for the second MRI examination. ***P < .001, **P < .01, 2-way ANOVA followed by Bonferroni multiple comparison test compared with WT mice or Kng^−/− mice; ns indicates not significant.