Igs of 1152 tumor subclones retain self-reactivity and antigen binding. (A) HEp-2 IFA staining pattern of tumor subclone Igs (2E12, 6C12, 4B11, 1G2, and 1E9) were obtained through rescue fusion of cells from the tumor biopsy of patient 1152; 1152 corresponds to the recombinant tumor Ig from patient 1152. Bars represent 25 μm. (B) Immunoblot for myoferlin in immunoprecipitations from lysate of 293T cells transfected with recombinant myoferlin-HA construct. Ly indicates lysate; B, lysate IP with protein G beads only; and 0516, an unrelated tumor Ig. (C) Tumor subclone Igs were tested for binding to recombinant myoferlin by ELISA. Recombinant Myoferlin-HA protein was immobilized using anti-HA antibodies on lysate from transfected 293T cells. Data shown are representative of at least 2 independent experiments.