Figure 5
![Figure 5. Prothrombinase binding to PS-expressing RBCs. WB was drawn via phlebotomy into 0.1 mg/mL of CTI, centrifuged at 150g, and then washed and treated with NEM and ionophore and the indicated reagent, as described in “Methods.” Treated cells were diluted to 2 × 106 cell/mL in RBC wash buffer and examined in a flow cytometer. Fluorescence gates were set using nonimmune IgG-FITC (A). PS exposure was detected with FITC-EGRck-fXa either in the absence (B) or presence (C) of saturating fVa. PS exposure was detected on untreated washed RBCs with FITC-EGRck-fXa/fVa (D [histogram] and E [scatter plot with PE-cd235a]).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/120/18/10.1182_blood-2012-05-427856/4/zh89991298490005.jpeg?Expires=1724917055&Signature=Y~1h13oHZNc5Vcl3kZvzIHVZXEqLih-VDjbEUoDw7worhRal-hcmp6CxIrvq9PxOIfu751XV8AN33MeAum6n5mlFRwgNTQH4vHnhwwFOkjBxi5AdE86W8b5lufcsWU2xpRzG5HkC8h6KL6wBt73YJ4X~8QWg81Zm8sz86vkwJqsfNXx8q7FmAWe~saHqu8XyAdDW1DwnCAFzObS-uz~O8iKp7DZrCuJu0yUiuqXlUdzpQ~4Frgi~WmososFnwhsVuDMyQOg5qtqF0Z63LOFENtNX7liCxlogZx-7Kicm27-xWxu0jqGGJeUDRMQ-EGd2VzwATLnCtBs8N0RKUEEYXw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Prothrombinase binding to PS-expressing RBCs. WB was drawn via phlebotomy into 0.1 mg/mL of CTI, centrifuged at 150g, and then washed and treated with NEM and ionophore and the indicated reagent, as described in “Methods.” Treated cells were diluted to 2 × 106 cell/mL in RBC wash buffer and examined in a flow cytometer. Fluorescence gates were set using nonimmune IgG-FITC (A). PS exposure was detected with FITC-EGRck-fXa either in the absence (B) or presence (C) of saturating fVa. PS exposure was detected on untreated washed RBCs with FITC-EGRck-fXa/fVa (D [histogram] and E [scatter plot with PE-cd235a]).
