γ camera quantification of 111-Indium–labeled eosinophils. (A) Anterior γ camera images at 5 minutes and 40 minutes after reinjection, showing accumulation in the right lung (RL), left lung (LL), liver (L), and spleen (S). (B) The distribution of radioactivity over 72 hours for the right lung (blue), liver (green), spleen (black), bone marrow (purple), and eosinophil recovery (red) after reinjection of 111-Indium–labeled eosinophils. (C) The distribution of radioactivity over 40 minutes for lungs, liver, spleen, bone marrow, and right ventricle after reinjection of 111-Indium–labeled eosinophils. (D) The final distribution of eosinophils within the liver and spleen at 48 hours. Radioactivity in other sites was calculated by subtracting the liver, spleen, lung, and blood activity values from the total radioactivity injected. The value obtained may reflect regions, such as the bone marrow, which are difficult to fully quantify on images of the upper body alone. Data represent the mean ± SEM of 6 independent experiments. *P < .05, compared with 40-minute liver values (1-way ANOVA test).