HHV8-specific T-cell frequencies in posttransplant patients. Specific IFNγ-enzyme-linked immunosorbent spot assay (IFNγ-ELISPOT) monitoring, either for lytic (orfK8.1; ▴) or for latent (orf73/LANA; ○) HHV8-derived antigens, was applied to assess HHV8-specific T-cell frequencies in our series of 15 PT-KS patients, either at PT-KS diagnosis or after remission, and in 2 PT-LPD patients (PEL or MCD; gray symbols), as well as in 5 HHV8-seropostive posttransplantation asymptomatic carriers (PT-AC). The Mann-Whitney nonparametric test was used to compare groups.