Effect of Psgl-1 antibody treatment on SCD. Representative intravital microscopy images of venules from Hbb^hβs/hβs BMT mice that received either IgG (A) or PSGL-1 (B) Ab injections. Number of rolling (C) or attached (D) cells per mm. Transcription level of CD68 (E) and TNF-α (F) of liver tissues. Panels G-I are levels of circulating adhesion molecules, (sE-sel, sP-sel, and sVCAM-1). Representative micrographs of liver sections from Hbb^hβs/hβs BMT mice receiving IgG, (J,M); or PSGL-1 Ab injection (K,N). Panels J and K are iron staining, panels M and N are H&E staining. (L) Quantification of iron deposition and (O) % of necrotic area from liver sections. *P < .05. **P < .001. N = 4-5 per group. Magnification is 200×, bar = 100 μm.