TSP1 triggers vaso-occlusive crises in SAD mice. We injected recombinant TSP1 (1 mg/kg) intravenously to wild-type (WT; blue) and SAD transgenic mice (brown) and characterized vaso-occlusions in kidneys by echo-Doppler recording of hemodynamic parameters. (A) We determined mean blood flow velocity (cm/s) in the right renal artery, before (none), or within 1 to 5 minutes, or between 5 and 20 minutes of TSP1 injection (*P < .05 vs control, none). (B) Representative echo-Doppler waveforms in the renal artery of WT and SAD mice before and after injection of TSP1. We also determined the heart rate (C; beats per minutes) and calculated the cardiac output (D; mL/min) from recording in the pulmonary artery. (E) Histologic analysis of wild-type and SAD kidneys 5 minutes after TSP1 injection by Masson trichrome. Black arrows show areas of vascular congestion and erythrocyte deposits.