Gene expression profiling and GSEA of KSLs. (A) Venn diagram showing numbers of genes differentially expressed between RUNX1(41-214) versus control and Runx1-deficient versus control and the number of genes in common between the 2 groups. (B) Hierarchical clustering of genes in common between RUNX1(41-214) and Runx1-deficient cells (nominal P < .05; fold change > 2). (C) GSEA of correlation between aging signature genes and genes differentially expressed in RUNX1(41-214) versus Runx1-deficient cells. (D) Genes from the aging signature gene set show differential expression between RUNX1(41-214) versus Runx1-deficient cells. (E) Analysis of relative gene expression by quantitative RT-PCR in RUNX1(41-214) KSL cells compared with control MigR1 KSL cells. (F) Immunoblot analysis of bone marrow lineage-negative cells expressing RUNX1(41-214). 41 indicates RUNX1(41-214) KSL; Runx1 KO, Runx1^{floxed/floxed} MxCre^+/− KSL; and CON, Runx1^{floxed/floxed} MxCre^−/− KSL.