Characterization of AAMφ-derived eicosanoids. (A) Quantification of arachidonic acid (AA) metabolites in 12-hour cultures of purified macrophages (top) and in peritoneal lavages (bottom). (B) PCA scores plot for 12-hour cultures of purified macrophages. (C) PCA loading plot for panel B, showing how individual eicosanoids contribute toward the primary and secondary principal (x and y, respectively) axes. (D) Pie chart showing the breakdown in the production of cyclo-oxygenase metabolites between WT-NeMφ and IL4Rα^−/−-NeMφ. (E) Schematic of the AA cascade reproduced using VANTED. Square boxes with red text represent genes; and rounded boxes with black text, measured metabolites. Black arrows indicate enzymatic reactions; and red arrows, auto-oxidation. Relative expression values (normalized such that the total over all 3 conditions = 1) are beneath the associated gene/metabolite. WT B malayi (left box) IL4Rα^−/−B malayi implanted (center), and WT thioglycollate (right). (F-G) PCA scores and loading plot as in panels B and C for the peritoneal lavages.