Overall gene expression and differential expression analysis. (A) Number of expressed genes in macrophage populations where expressed is considered as at least 1 read mapping to a gene in all 3 replicates of a condition. (B) Unsupervised, hierarchical clustering of individual lanes demonstrating discrete clustering of biologic replicates. (C) Summary of DE genes (P < .01) in each pairwise comparison showing the total number of DE genes (inner), and a breakdown showing the direction of differential expression for both moderately (log 2-fold change ± 2-4) and highly (log 2-fold change > 4) DE genes.