Profile of NK cells derived from a LAD-III patient. (A) Family pedigree of the LAD-III patient. Striped symbols represent the heterozygous parents; full gray symbols, LAD-III patients; and the X symbol, the sibling who unfortunately died. (B) Freshly derived (2 left columns) and IL-2–activated (2 right columns) NK cells were obtained from the LAD-III patient and from a healthy control (indicated above the histograms). NK cells were stained for the expression of various activating receptors (indicated at the left part of the figure). Numbers in the figure indicates median fluorescence intensity of the gray histograms. (C) NK cells from the LAD-III patient and from a healthy control (indicated in the left part of the figure) were stained for the expression of LFA-1. (B-C) Black empty histogram represents the staining of the cells with a control mAb; gray empty histogram, specific staining. Figure shows 1 representative experiment of 3 performed.