Cellular distribution of iron after 2 weeks of PR65 injections for the treatment of established iron overload. Tissue sections correspond to the animals analyzed in Figure 6, with representative images shown. Horizontal bars indicate 400 μm (10 ×) and 100 μm (40 ×). Enhanced Perls stain confirmed that splenic macrophages and duodenal enterocytes retained iron in PR65-treated but not in solvent-treated mice. Compared with solvent-treated controls, less intense iron staining was observed in the liver of mice treated with PR65. No consistent differences between solvent and PR65-treated mice were seen in sections of the heart (not shown).