The hypoferremic effect of PR65 in iron-loaded hepcidin knockout mice. (A) Twenty-four hours after a subcutaneous injection, PR65 induced a dose-dependent decrease in serum iron. Mean values and standard deviations are shown, n = 3-5 mice per point (#P = .005 and P = .004, *P < .001). (B) The time course of hypoferremia induced by a subcutaneous injection of 100 nmol of PR65. Mean and standard deviations are shown, n = 4-6 mice per point (#P = .008, *P < .001).