Minihepcidin PR65 and its activity in wild-type mice. (A) Structural formula of PR65. Ida indicates iminodiacetic acid; Dpa, diphenylalanine; bhPro, β-homo proline; and bhPhe, β-homo phenylalanine. (B) Serum iron in wild-type C57BL/6 mice 4 hours after intraperitoneal injection of solvent, native hepcidin or PR65 (n = 4-8 in each group; **P = .01, *P = .005). (C) Serum iron in wild-type C57BL/6 mice 4 hours after intraperitoneal or subcutaneous injection of 20 nmol of PR65 (n = 4 in each group; *P = .007, **P = .04). In panels B and C, the bars represent mean values and error bars standard deviations.