Figure 2
GP1a prevents DC matrigel migration. DC were treated with CCP with or without GP1a (5 μM or 1 μM) for 24 or 48h (A) or MMP-9 inhibitor (M9i) for 48h (B) and 105 cells were placed in upper Transwell chambers coated wtih Matrigel (100 μg). The bottom chambers were filled with serum free medium with or without CCL19 (100 ng/ml). 3h later migrated cells were collected from the lower chambers and counted by FACS. (C) DC were treated as in A and Mmp9 mRNA expression was determined by qRT-PCR. Data are representative of 3 independent experiments.
