In utero WAT-KLS cells transplantation. Liver transplantation of 2 × 10⁴ WAT-KLS cells was performed in utero in embryos at embryonic day 13. Total chimerism was quantified in the WAT, blood, BM, thymus, and spleen 2 months after birth (A). Percentage of total cells expressing either the donor (CD45.1) or the host (CD45.2) cell marker. (B) Analysis of mature immune cells in the CD45.1⁺ population by flow cytometry. The percentages of CD45.1⁺ cells expressing lineage markers are indicated. Dot plots are representative of 3 independent animals.