Quantification of chimerism in mature immune cells in WAT after reconstitution of lethally irradiated mice. Lethally irradiated CD45.2 mice were coinjected with 10³ CD45.1⁺ KLS cells sorted from WAT (black symbols) or BM (open symbols) and 2.10⁵ total BM cells isolated from CD45.2 mice. Four months after transplantation, T lymphocytes (CD4⁺ or CD8⁺ gated on CD3ϵ⁺; A), B lymphocytes (CD19⁺/B220⁺; C), NK cells (NK1.1⁺/CD3ϵ⁻; E), macrophages (CD11b⁺/F4/80⁺; G), and mast cells (FcϵRI⁺/CD117⁺; I) in WAT were identified by flow cytometry, and the proportion of CD45.1⁺ cells was analyzed in each population (B, D, F, H, and J). Dot plots are representative of 6-8 independent experiments.