Figure 4
In vivo cell imaging of immune cells derived from WAT-KLS cells. 1000 GFP+ WAT-KLS cells were IV coinjected with 2.105 GFP− total BM cells into lethally irradiated CD45.2 syngenic mice. One month after injection, videos of intrafemoral BM, spleen, and WAT were performed to track GFP+ cells. (A) Frames of 20-35-second videos acquired at the surface of these organs (spleen and WAT) or inside the length of the femur showing the GFP+ cells observed. Results are representative of 3 independent animals. (B) Successive pictures of 15 mm of WAT observed with the confocal macroprobe.
