Quantification of chimerism after reconstitution of lethally irradiated mice. Lethally irradiated CD45.2 mice were coinjected with 10³ sorted KLS cells isolated from either the WAT or BM of CD45.1 mice and 2.10⁵ total BM cells were harvested from CD45.2 mice. Total chimerism was quantified in hematopoietic organs and peripheral blood (A) and in WAT and inguinal LNs (B-C) in primary recipients 4 months after injection. Chimerism was expressed as a percentage of CD45.1⁺ cells belonging from WAT-KLS cells (black symbols) or BM-KLS cells (open symbols) of total CD45⁺ cells in each organ. Dot plots are representative of 6-8 independent experiments.