Characterization of WAT-KLS cells. (A) Five-color flow cytometry analyses were performed on KLS cells (gated on singlet, viable c-Kit⁺/Lin⁻/Sca-1⁺). Dot plots are representative of 6-7 independent experiments. (B) Expression of transcription factors involved in hematopoietic commitment was measured by quantitative RT-PCR performed on sorted WAT-KLS cells (solid line) and BM-KLS cells (dotted line). Mean Ct values of 4-6 independent samples are plotted on the graph. Transcription factors with Ct values greater than 33 (gray zone) are considered to be nonexpressed in the cell population.