miR-30b and miR-30c regulate DLL4.DLL4 (A-C-D) and SELE (A) mRNA expression, as measured by qRT-PCR, in LECs (A-C) or HUVECs (D) transfected with mimics (A) or infected with lentiviruses (C-D). DLL4 protein expression, as measured by Western blotting, in LECs transfected with mimics (B), infected with lentiviruses (C), or transfected with inhibitors (E). Values indicate intensity of DLL4 antibody ECL plus signal normalized to GAPDH antibody ECL signal. (A) Expression is relative to nontargeting control (NTC) mimic (means + SEM, n = 4). (B) Top panel: Representative Western blots, 20 minutes' exposure of DLL4 blot and 1-second exposure of GAPDH blot. Bottom panel: intensity is relative to NTC mimic (means + SEM, n = 3). (C) Top panel: Representative Western blots, 10 minutes' exposure of DLL4 blot and 1-second exposure of GAPDH blot. Bottom panel: expression is relative to empty vector, pSIN_MCS (means + SEM, n = 4). (D) Expression is relative to empty vector, pSIN_MCS (means + SEM, n = 3). (E) Top panel: representative Western blots, 30 minutes' exposure of DLL4 blot and 1-second exposure of GAPDH blot. Bottom panel: Intensity is relative to NTC inhibitor (means + SEM, n = 2). (F) Reporter assay indicating the response of WT or mutant (mut) DLL4 3′UTR to exogenous miR-30b and miR-30c (means + SEM, n = 3). Firefly expression was normalized to renilla expression to give the relative light units (RLU), which are shown relative to NTC mimic. MT01 is a control reporter, lacking a 3′UTR sequence but containing the firefly and renilla luciferase genes. Related statistically significant values are indicated by horizontal bars. In all panels, statistical significance denoted by *P < .05; **P < .01; ***P < .001.