Differential accessibility between leukemic subtypes. (A) Overview of Nlalll-identified accessible regions at the RPS17 and CR1 genomic regions in APL (purple) as well as in NB4 cells (blue). (B) Correlation of accessible regions (high, medium, and low) and a set of randomly chosen regions in proliferating NB4 cells with the accessibility library in an APL patient sample. Box plot showing the normalized tag count for NB4 proliferating cells and for an APL patient sample (pz258) in accessible regions as defined in NB4 cells. (C) Enrichment of accessible regions defined by NA-seq in CD34⁺ cells within NA-seq defined NB4 accessible regions. Enrichment was investigated from the middle of the NB4 NA-seq dataset to a distance of ± 5 kb. (D) Overlap of accessible regions defined by NA-seq in APL (NB4 cells) and t(8;21) AML (SKNO-1 cells). Left panel: HpaII data. Right panel: NlaIII data. (E) Correlation of accessibility between different AML patient samples. In scatter plots, the NA-seq tag count was plotted in windows of 1.2 Mb over the whole genome (blue). Differential accessible regions between M1 and M3 patients and bound by PML-RARα are highlighted (brown). The correlation coefficient between pairs of datasets is depicted in the top left corner of each scatter plot.