Effect of mAbs to VWF in thrombus formation in mice expressing huVWFmuA1. Four- to 5-week-old VWF^−/− mice were hydrodynamically injected with 150 μg of pLIVE huVWFmuA1 cDNA by hydrodynamic injection. Four days later, mice were injected with 100 μg/mouse of mAb 9 (recognizing RGD-motif), mAb 203 or mAb 505 (both recognizing A3 domain), and thrombosis was induced by 7.5% FeCl₃ exposure 30 minutes after mAb injection. The time necessary to form a thrombus of > 30 μm in veins (A) and in arterioles (B) was recorded. Statistical analysis was done using Wilcoxon-Mann-Whitney rank-sum test. Time to occlusion in veins (C) and arterioles (D) was also measured. NS indicates not significant. Results from individual mice are represented by a dot, and a horizontal line represents the average occlusion time for each group. Statistical analysis was done with Fisher exact 2-sided test.