Comparative effect of huVWFmuA1 versus muVwf in thrombus formation in vivo. Four-week-old VWF−/− mice received 150 μg of pLIVE plasmid containing either huVWFmuA1 (n = 8) or control muVwf (n = 7) cDNA by hydrodynamic injection. Four days after, in vivo thrombosis was assessed after exposure of mesenteric vessels to 7.5% FeCl3 for 5 minutes. Platelets were labeled in vivo through injection of rhodamine 6G before mesentery dissection. Platelet accumulation was recorded during 40 minutes or until final occlusion of the vessel. (A) Photographs of arterioles from muVwf and huVWFmuA1-expressing mice at different time points after FeCl3 exposure. (B) Time necessary to form a thrombus > 30 μm in veins or arterioles of mice expressing muVwf or huVWFmuA1. *P < .05 in arterioles of huVWFmuA1-expressing mice compared with muVwf-expressing mice. Statistical analysis was done using Wilcoxon-Mann-Whitney rank-sum test. (C-D) Occlusion time in veins (C) and arterioles (D) of muVwf and huVWFmuA1-expressing mice. Result from an individual mouse is represented by a dot, and a horizontal line represents the mean time of occlusion for each group. Statistical analysis was done with Fisher exact 2-sided test.
