Analysis of the capacity of the different human-murine VWF chimeras to correct bleeding time of VWF^−/− mice. Eight-week old VWF^−/− mice received 150 μg of pCDNA6 containing the different constructs by hydrodynamic injection. Bleeding tests were done 24 hours after injection by the tail clip assay. Observation was stopped at 600 seconds when bleeding did not cease. Each dot represents an individual mouse, and the horizontal line represents the mean bleeding time per group. Statistical analysis was done using Fisher exact 2-sided test by comparison with muVwf. NS indicates not significant.