Construction and characterization of the different human-murine VWF chimeric constructs. (A) Schematic representation of the different VWF constructs with indications of human to murine amino acid changes introduced in human VWF A1 domain. Point mutations and insertions of murine amino acid sequences (shaded area) were done by site-directed mutagenesis. (B) VWF antigen levels after hydrodynamic injection of 150 μg of pCDNA6 containing the different constructs in VWF^−/− mice. VWF plasma levels were quantified by ELISA 24 hours after hydrodynamic injection. (C) Multimeric profile of VWF expressed by VWF^−/− mice after hydrodynamic injection of the various constructs. Plasma samples were analyzed using SDS/2% agarose gel electrophoresis.