Loading Eμ-myc tumor vaccine with β-ManCer gives prolonged therapeutic effect. WT mice were challenged with 1 × 105 Eμ-myc 299 (A) tumor cells and 5 days later treated with single administration of irradiated, Eμ-myc tumor cells pulsed overnight with 500 ng/mL of the indicated glycolipids. (A) Mean ± SEM (n = 5) tumor burden in blood of Eμ-myc 299 tumor challenged mice after vaccine treatment. Middle and right panels: Individual mouse tumor burden at day 19 and 26 time point from panel A, with statistical evaluation. (B) Serum cytokine levels from Eμ-myc tumor-bearing WT mice 24 hours after vaccination with tumors loaded with the indicated glycolipids. ***P < .0005 (unpaired t test). **P < .005 (unpaired t test). *P < .05 (unpaired t test). ns indicates not significant. Two independent experiments were performed.
